Composite

Part:BBa_K3242006:Design

Designed by: Lui Suzuki-Williams   Group: iGEM19_UGA   (2019-10-19)


GAL4BD - VP16


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 343
    Illegal SapI site found at 198


Design Notes

This sequence was optimized for use in gateway cloning, agrobacterium, and transfection in Nicotiana benthamiana.

Source

The GAL4BD-VP16 part was designed by combining the GAL4BD sequence (BBa_K3242004) and the VP16 transactivating domain (BBa_K3242005) sequence. GAL4BD is derived from the yeast genome, and VP16 is derived from the Herpes simplex viral genome.

References

1. “Transcription Factor GAL4.” Transcription Factor GAL4 - an Overview | ScienceDirect Topics, 

  www.sciencedirect.com/topics/neuroscience/transcription-factor-gal4.

2. Fischer, Janice A., et al. “GAL4 Activates Transcription in Drosophila.” Nature, vol. 332, no. 6167, 1988, pp. 853–856., 

  doi:10.1038/332853a0.

3. Hirai, Hiroyuki, et al. “Structure and Functions of Powerful Transactivators: VP16, MyoD and FoxA.” The International Journal of Developmental Biology, vol. 54, no. 11-12, 2010, pp. 1589–1596., 

  doi:10.1387/ijdb.103194hh.

4. Nygaard, Kayla. “Yeast 2-Hybrid.” Washington University School of Medicine in St. Louis, 2018, 

  genetics.wustl.edu/bio5491/files/2018/02/KRN-Yeast-2-Hybrid.pdf.